Intermediate filament proteins immunologically related to desmin in astrocytes: A study of chicken spinal cord by two-dimensional gel electrophoresis and immunoblotting |
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| Authors: | D Dahl PhD C J Crosby A Bignami |
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| Institution: | (1) Spinal Cord Injury Research Laboratory, Veterans Administration Medical Center, 02132 Boston, MA;(2) Department of Neuropathology, Harvard Medical School, 02115 Boston, MA;(3) Department of Pathology, Harvard Medical School, 02115 Boston, MA;(4) Research Service (151), Veterans Administration Medical Center, 1400 VFW Parkway, 02132 West Roxbury, MA |
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| Abstract: | Co-migration experiments by two-dimensional SDS-PAGE using chicken spinal cord extracts and desmin purified from chicken gizzard showed that desmin is not present in spinal cord. However, by the immunoblotting procedure, desmin antibodies recognized 3 spinal cord antigens with different molecular weights and isoelectric points than desmin and the glial fibrillary acidic (GFA) protein. These antigens which also reacted with GFA protein antibodies were not identified in chicken gizzard extracts. The reactivity of the antigens with a monoclonal antibody recognizing an epitope common to most intermediate filament proteins (1) suggests that immunostaining of astrocytes with desmin antibodies (2, 3) is due to the presence of new intermediate filament proteins immunologically related to desmin.Special issue dedicated to Dr. Paola S. Timiras |
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| Keywords: | Astrocytes desmin GFA protein immunoblotting intermediate filaments |
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