水稻幼穗培养高效再生系统的建立

本文测试了不同基因型幼穗取材时期、消毒方式、4℃下处理时间、愈伤组织诱导、分化及生根条件等对水稻幼穗培养再生成株的影响，实验结果表明，在优化条件下，籼稻的绿苗分化率在85%以上，成苗率115%左右；粳稻的绿苗分化率在90%以上，成苗率130%左右；粳型广亲和的绿苗分化率、成苗率分别达到85%和115%以上。由此建立了一套高效、可靠、重复性好的水稻组织培养再生系统，为水稻遗传转化的顺利进行奠定了基础。

Efficient Regeneration from In Vitro Culture of Young Panicles of Rice (Oryza sativa L.)

Factors affecting the regeneration of rice plants from young panicles , such as developmental stage of young panicles in different genotypes, sterilizing methods, duration of 4℃ treatment, induction, differentiation and rooting conditions, etc., were investigated. The results showed that the optimal conditions for different genotypic rice were as following: after the best developmental stage ( Ⅲ~Ⅳ of indica,Ⅰ~Ⅱ of japonica,Ⅴ of wide compatible variety, WCV) of young panicles was treated at 4℃ within 5 days, the materials were sterilized with 0.5% potassium hypochlorite for 20 minutes, cultivated in YS-Y medium about 20 days to induce the calli , then transferred to YS-YF、YS-F medium about 15 days to regenerate shoots and YS-SG medium for 1~2 weeks to induce the roots. Under the optimal conditions, the frequency of green shoot differentiation was a little more than 85% (indica), 90% (japonica), 85% (WCV), the frequency of root regenerated was about 115% (indica), 130% (japonica), 115% (WCV), respectively. Therefore a high efficient regeneration system from in vitro culture of young panicles of rice was established for genetic transformation of rice.