一株高效广谱染料降解细菌的分离鉴定及其脱色特性初探

从土壤样品中分离到一株高效染料脱色菌株N-4,根据形态学特征及16S rDNA基因序列分析,该菌株初步鉴定为Leucobacter sp.。利用表面响应法(RSM)对菌株N-4脱色活性深蓝K-R的主要因素进行优化,实验结果表明,菌株N-4脱色K-R的最优条件为:湿菌量10 g/L,染料浓度222 mg/L,硫酸铵1.5 g/L,果糖3.5 g/L,最佳脱色率为100%。此外,实验证明其对多种染料均具有较高的脱色效率。同时,考察了金属离子对染料脱色效率的影响,其中K+、Ca2+、Mg2+、Ba2+、Mn2+等对脱色具有促进作用,而Ni2+、Cu2+、Hg2+对脱色具有明显的抑制作用。

Isolation and characterization of a dye-colorizig bacterial strain

A new dyes-decolorizig bacterial strain N-4 was isolated from soil sample. It was indentified as Leucobacter sp. by 16S rDNA gene sequence analysis. The optimal conditions for the decolorization of the Reactive Dark Blue K-R determined by response surface methodology (RSM) were as follows: 10 g/L of strains N-4, 222 mg/L of initial dye concentration, 1.5 g/L of (NH3)2SO4 and 3.5 g/L of fructose. It was shown that about 100% of 222 mg/L dye could be decolorized within 10 h under these conditions. Besides, strain N-4 could decolorize 9 kinds of dyes effectively. And K+, Ca2+, Mg2+, Ba2+, Mn2+ could improve the decolorizaion, while Ni2+, Cu2+ and Hg2+ inhibite the decolorization obviously.