Expression profiling in HcrVf2-transformed apple plants in response to Venturia inaequalis |
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Authors: | Roberta Paris Valentina Cova Giulia Pagliarani Stefano Tartarini Matteo Komjanc Silviero Sansavini |
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Institution: | (1) Department of Fruit Tree and Woody Plant Sciences, University of Bologna, viale Fanin 46, 40127 Bologna, Italy;(2) Genetics and Molecular Biology Department, IASMA Research Centre, Via E. Mach 1-38010 S., Michele all’Adige (TN), Italy |
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Abstract: | Apple scab resistance is one of the most well-characterized plant–pathogen interactions in a woody plant species. While the
HcrVf2 gene from the wild apple Malus floribunda 821 has proved capable of conferring scab resistance to the susceptible cv. Gala after genetic transformation, its identification
represents only the first step in understanding the molecular mechanisms and, hence, the network of genes underlying the defence
response. We used a PCR-based suppression subtractive hybridization to identify apple genes that are differentially expressed
after Venturia inaequalis inoculation. Subtractive hybridization was performed between cDNA from challenged leaves of HcrVf2-resistant transgenic Gala and susceptible cv. Gala plants. A library of 523 unigenes was constructed and characterized by
assigning a putative function via comparison with public databases. This set of pathogen-modulated apple genes includes many
defence-related genes and is therefore an important source of information for understanding the molecular basis of the Malus–V. inaequalis interaction.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Apple scab Defence Subtracted library Malus × domestica |
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