Establishment of cell strains from human urothelial carcinoma and their morphological characterization |
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Authors: | Takahide Kakuya Takashi Yamada Masayuki Yokokawa Tadakazu Ueda |
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Institution: | (1) Department of Urology, Tokyo Medical and Dental University School of Medicine, Yushima, Bunkyo-ku, 113 Tokyo, Japan;(2) Department of Pathology, Dokkyo University School of Medicine, Kitakobayashi 880, 321-02 Mibu, Tochigi, Japan |
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Abstract: | Summary We have examined the conditions for cultivation of enzymatically dispersed cells from 34 human urothelial transitional cell
carcinomas (TCC) of various types. By employing two culture methods, stationary and tapping suspension, and by using the synthetic
medium DM 160 supplement with human umbilical cord serum and fetal bovine serum, six cell strains were established. In two
strains the tapping suspension culture method was suitable for growth of highly malignant cancer cells that detach easily
from the glass surface in stationary cultures. Each of the six cell strains has been maintained in culture for over 30 months
with repeated subcultures of 32 to 128 times. The histopathological features of the original TCC were three differentiated
papillary types and three anaplastic nonpapillary types. In two cell strains from TCC with low malignancy, however, the cancer
masses that formed in nude mice differed from the original TCC in which they became more malignant, and one cell strain resembled
the original TCC closely. In three stationary culture cell strains the epithelial nature was demonstrated by the presence
of desmosomes and tonofilaments. In one cell strain only tonofilaments were present. In two tapping suspension culture cell
strains the presence of desmosomes was not shown clearly, but fine tonofilaments were observed in one cell strain.
This work was supported in part by Grants 5319 and 5322 in aid for cancer research from the Ministry of Health and Welfare,
Japan. |
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Keywords: | primary culture tapping suspension culture cell strains human urothelial carcinoma |
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