Synthesis of the arabinose operon regulator protein in a cell-free system |
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Authors: | Huey-Lang Yang and Geoffrey Zubay |
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Affiliation: | (1) Department of Biological Sciences, Columbia Univesity, New York City, New York |
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Abstract: | Summary A DNA-directed cell-free system which synthesizes L-ribulokinase coded by the L-arabinose operon, ara OIBAD, has been developed. L-arabinose is required for the expression of this operon and, in addition, cyclic AMP and guanosine-tetraphosphate are needed for optimal synthesis. The ara C gene product is also required and can be supplied either by de novo synthesis in the cell-free system or added back from extracts of whole cells. Evidence is presented that the C gene product is highly susceptible to protease attack. |
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