Growth patterns and alkaloid accumulation in hairy root and untransformed root cultures of Datura stramonium |
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Authors: | Baíza Ana María Quiroz Adriana Ruíz José Alfredo Maldonado-Mendoza Ignacio |
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Institution: | (1) Unidad de Biología Experimental, Centro de Investigación Científica de Yucatán, Apdo. Postal 87, Cordemex, 97310 Yucatán, México |
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Abstract: | The aim of this work was to study the possible relationship between alkaloid production and growth measured as: biomass increase
and cellular division frequency, in Datura stramonium in vitro root cultures (hairy root and normal cultures). A comparison
of growth values on a fresh and dry weight basis showed that there were differences between transformed and non-transformed
lines. The differential growth between lines occurred due to a real biomass increase and not because of water accumulation.
On the other hand, the rate of cell division showed a similar pattern for all lines studied. Therefore, the differences in
growth are not due to different cell division rates, nor to the presence of larger meristems, but to the development and growth
of lateral roots and the presence of active intercalary meristematic zones in each line. The maximum alkaloid production occurred
when the cultures were not growing. This suggests an inverse relationship. Finally, the data support a specific model of growth
at the level of cell division in root cultures which has not been described before in the literature.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | cell division hyoscyamine meristem size root growth scopolamine |
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