Magnesium-dependent non-specific binding of [125I]prolactin to myelinated tracts in the rat central nervous system |
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Authors: | L P Mangurian R J Walsh B I Posner |
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Institution: | Department of Anatomy, George Washington University Medical Center, Washington, D.C. |
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Abstract: | An in vitro autoradiographic assay was used in identifying a magnesium-dependent, non-specific binding of 125I] prolactin to myelinated fiber tracts in the rat brain. Frozen tissue sections were incubated for 18 h at 4 degrees C in media which included 125I]prolactin alone or with a 500 fold excess of unlabelled prolactin. Magnesium in the incubation medium caused a non-specific binding of radiolabelled prolactin to the myelinated fiber tracts in the brain. In contrast, calcium did not facilitate prolactin non-specific binding to myelin. Hence, calcium should optimize the detection of specific prolactin binding sites in the brain by in vitro autoradiographic or radioreceptor assays. |
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