Periodate oxidation studies in the elucidation of the structures of sialic acid containing oligosaccharides |
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| Authors: | W D Gathmann D Aminoff |
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| Affiliation: | 1. Department of Internal Medicine (Simpson Memorial Institute), University of Michigan, Ann Arbor, MI 48109 U.S.A.;2. Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109 U.S.A. |
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| Abstract: | The structures of sialo-oligosaccharide alditols as determined by 1H-NMR spectrometry together with methylation analysis did not correspond with those derived previously from quantitative periodate oxidation data alone. Possible causes of the discrepancy were explored in the periodate oxidation methodology. No free sialic acid was released by the acidity of the periodate in the course of oxidation at pH 4.5. The anionic properties of the sialic acid residues were therefore utilized to separate the periodate oxidation products and thereby establish the position of the sialic acid in the oligosaccharide chain. |
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| Keywords: | To whom correspondence should be addressed. |
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