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Oxaloacetate and malate production in engineered Escherichia coli by expression of codon-optimized phosphoenolpyruvate carboxylase2 gene from Dunaliella salina |
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| Authors: | Soohyun Park Kwang Suk Chang EonSeon Jin Seung Pil Pack Jinwon Lee |
| Institution: | 1. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea 2. Department of Life Science and Research Institute for Nature Science, Hanyang University, Seoul, 133-791, Republic of Korea 3. Department of Life Science, Hanyang University, Seoul, 133-791, Republic of Korea 4. Department of Biotechnology and Bioinformatics, Korea University, Jochiwon, Chungnam, 339-800, Republic of Korea |
| Abstract: | A new phosphoenolpyruvate carboxylase (PEPC) gene of Dunaliella salina is identified using homology analysis was conducted using PEPC gene of Chlamydomonas reinhardtii and Arabidopsis thaliana. Recombinant E. coli SGJS115 with increased production of malate and oxaloacetate was developed by introducing codon-optimized phosphoenolpyruvate carboxylase2 (OPDSPEPC2) gene of Dunaliella salina. E. coli SGJS115 yielded a 9.9 % increase in malate production. In addition, E. coli SGJS115 exhibited two times increase in the yield of oxaloacetate over the E. coli SGJS114 having identified PEPC2 gene obtained from Dunaliella salina. |
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