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Identification of plasma inactive renin as prorenin with a site-directed antibody
Authors:S J Kim  S Hirose  H Miyazaki  N Ueno  K Higashimori  S Morinaga  T Kimura  S Sakakibara  K Murakami
Institution:1. Institute of Applied Biochemistry, University of Tsukuba, Ibaraki 305, Japan;1. Pathology Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo 104, Japan;7. Peptide Institute, Inc., Protein Research Foundation, Ina, Minoh-shi, Osaka 562, Japan;1. Geo-environmental Engineering Group, Civil Engineering Department, Central Mindanao University, Bukidnon, Philippines;2. School of Minerals and Energy Resources Engineering, The University of New South Wales, Sydney, NSW, Australia;3. Materials Science Research Group, Physics Department, Central Mindanao University, Bukidnon, Philippines;4. Division of Sustainable Resources Engineering, Faculty of Engineering, Hokkaido University, Sapporo, Japan;5. Department of Biological Sciences, College of Science and Mathematics, Mindanao State University–Iligan Institute of Technology, Iligan City, Philippines;1. Laboratório de Neurociências, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil;2. Center for Experimental Models in Psychiatry, Department of Psychiatry and Behavioral Sciences, Medical School, The University of Texas Health Science Center at Houston, Houston, TX, USA;3. Laborátorio de Erros Inatos do Metabolismo, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil;1. Department of Experimental Physics 5, University of Würzburg, Würzburg, Germany;2. Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany;3. Fraunhofer Institute for Integrated Circuits (IIS), Development Center for X-ray Technology (EZRT), Würzburg, Germany;1. Los Alamos National Laboratory, Los Alamos, NM, USA;2. University of Michigan, Ann Arbor, MI, USA;3. KTH Royal Institute of Technology, Stockholm, Sweden;4. Air Force Research Laboratory, Kirtland AFB, NM, USA;5. Space Science Institute, Boulder CO, USA;6. New Mexico Consortium, Los Alamos, NM, USA;7. British Antarctic Survey, Cambridge, England, United Kingdom;1. Giresun University, Civil Engineering Department, Giresun 28000, Turkey;2. Metropolitan University of Tirana, Faculty of Engineering and Architecture, Tirana 1000, Albania;3. Jeddah University, School of Industrial Engineering Department, Jeddah 21577, Saudi Arabia;4. TUSSIDE/TUBITAK, Kocaeli 41401, Turkey
Abstract:A sequence-specific antibody that recognizes a portion of the prosegment of human renin precursor was raised and used to provide direct evidence that plasma inactive renin contains the prosequence of renal renin and is therefore probably prorenin rather than an inactivated form of previously active renin. The information may help not only to resolve a major controversy concerning the nature of inactive renin in human plasma but also to elucidate its exact physiological role.
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