Evidence for the occurrence of an alkaline proteinase in kidney cortex lysosomes |
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| Authors: | P Harikumar T Darshini V Ninjoor |
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| Institution: | Food Technology & Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay. |
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| Abstract: | A novel alkaline endoproteinase optimally active at pH 8.5 has been detected in highly pure preparations of buffalo kidney cortex lysosomes. The enzyme has been partially purified (90-fold) by solubilization with octylglucoside, acid precipitation and chromatography over DEAE sephacel and sepharose 6B. The alkaline proteinase, resistant to known inhibitors of lysosomal cathepsins is inhibited by soyabean trypsin inhibitor and phenyl methyl sulphonyl fluoride indicating that the enzyme is a serine proteinase. |
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