Unexpected activity of beta-cyclodextrin against experimental infection by Cryptosporidium parvum |
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Authors: | Castro-Hermida J A Freire-Santos F Oteiza-Löpez A M Ares-Mazás E |
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Institution: | Laboratorio de Parasitología, Departamento de Microbiología y Parasitología, Universidad de Santiago de Compostela, La Coru?a, Espa?a. |
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Abstract: | An unexpected activity of beta-cyclodextrin, an excipient used in pharmaceutical technology, was observed against Cryptosporidium parvum. The viability and infectivity of purified oocysts, exposed for 24 hr to beta-cyclodextrin (2.5% suspension), were evaluated by inclusion/exclusion of 2 fluorogenic vital dyes and a suckling murine model, respectively. Results of the viability assay showed a high proportion of nonviable oocysts (81.5%). The intensity of experimental infection, determined 7 days postinoculation by examination of intestinal homogenates, was significantly lower (P < 0.05) than in the control litters. The preventive and curative efficacies of beta-cyclodextrin suspension were also evaluated in experimentally infected neonatal mice. Infection was prevented when the suspension was administered 2 hr before inoculated oocysts and on days 1 and 2 postinoculation. |
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