华根霉合成活性脂肪酶液态发酵菌体形态调控的研究

华根霉(CCTCC M201021)膜结合脂肪酶在非水相中具有突出的催化酯合成的能力,在生物香料、生物柴油生产等工业应用中具有良好的前景.与许多丝状真茵形态影响其初级及次级代谢产物的生产相类似,华根霉在液态发酵中也会形成不同的茵体形态,显著影响合成活性膜结合脂肪酶的发酵水平.本研究以华根霉菌体形态及脂肪酶合成活性为指标...

Studies on morphologic regulation of Rhizopus chinensis in submerged fermentation of lipase with synthetic activity

Membrane-bound lipase with high activity produced by Rhizopus chinensis （CCTCC 2010021 ） could catalyze the synthesis of ester in non-aqueous phase and had potential applications in the production of flavor and fragrance compounds, biodiesel, and so on. Similar to other filamentous fungi, Rhizopus chinensis would form different morphologies in submerged fermentation, which influenced the production of membrane-bound lipase with synthetic activity obviously. Based on mycelial morphology and synthetic activity of Rhizopus chinensis lipase, the influences of fermentation conditions on the mycelial morphologies and lipase synthetic activity were investigated. The results showed that the medium volume, rotation speed and baffled flask remarkably influenced the Rhizopus chinensis morphology and the higher lipase synthetic activity could be obtained by clump mycelia. The optimized fermentation conditions for clump mycelia were as follows： medium volume 20 mL/250 flask, 200 r/min, initial pH 5.5, inoculum 5×10^7 spores/mL. Under the optimized fermentation conditions, clump mycelia were formed and synthetic activity of Rhizopus chinensis lipase reached up to 1080 U/g.