|
|||||
|
|
| 甲基营养菌Methylobacterium sp.MB200中与L-丝氨酸耐受性相关基因的克隆与分析 | |
| 引用本文： | 周侃,唐咸来,蒋承建,宋修鹏,李俊芳,田丹丹,申佩弘. 甲基营养菌Methylobacterium sp.MB200中与L-丝氨酸耐受性相关基因的克隆与分析[J]. 工业微生物, 2011, 41(6): 38-42. DOI: 10.3969/j.issn.1001-6678.2011.06.008 |
| 作者姓名： | 周侃 唐咸来 蒋承建 宋修鹏 李俊芳 田丹丹 申佩弘 |
| 作者单位： | 1. 广西大学生命科学与技术学院,南宁,530005;微生物及植物遗传工程教育部重点实验室,南宁,530005;广西亚热带生物资源保护利用重点实验室,南宁,530005 2. 广西壮族自治区科学技术厅,南宁,530005 3. 广西大学生命科学与技术学院,南宁,530005 |
| 基金项目： | 第四十九批中国博土后基金,国家自然科学基金 |
| 摘 要： | 甲基营养菌MB200,能利用非C-C键低碳化合物生长并合成L-丝氨酸,对L-丝氨酸耐受浓度低,提高其在静息细胞反应系统中对L-丝氨酸的耐受能力可更好的提高L-丝氨酸的产量.利用质粒转座子pTnMod-RKm'构建了甲基营养菌MB200的突变体库,往培养基中添加20 mg/mL L-丝氨酸对突变体库中的突变体进行平板筛选... |
| 关 键 词： | Methylobacterium sp.MB200 突变体 L-丝氨酸 耐受性 基因克隆 |
Cloning and analysis of genes related to L-serine tolerance from Methylobacterium.sp MB200 |
|
| ZHOU Kan,TANG Xian-lai,JIANG Cheng-jian,SONG Xiu-peng,LI Jun-fang,TIAN Dan-dan,SHEN Pei-hong. Cloning and analysis of genes related to L-serine tolerance from Methylobacterium.sp MB200[J]. Industrial Microbiology, 2011, 41(6): 38-42. DOI: 10.3969/j.issn.1001-6678.2011.06.008 | |
| Authors: | ZHOU Kan TANG Xian-lai JIANG Cheng-jian SONG Xiu-peng LI Jun-fang TIAN Dan-dan SHEN Pei-hong |
| Affiliation: | 1. College of Life Science and Technology of Guangxi University; 2. The Key Laboratory of Ministry of Education for Microbial and Plant Genetie Engineering; 3. Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization;4. The Science and Technology Department of Guangxi; Nanning 530005, Guangxi, China) |
| Abstract: | Methylobacterium sp. MB200, can bioconvert the carbon compounds containing no carbon-carbon bonds to synthesize L-serine, its L-serine tolerance must be promoted to increase the yield of L-serine in resting cell reaction system. Using the plasmid pTnMod-Rkm＇, a partial mutant library of M. sp. MB200 was constructed. 177 strains showing better resistance to L-serine were obtained and then re-screened by 10 mg/mL concentration gradient. The results indicated that some mutants could grow on the medium with 40 mg/mL L-serine. The genomic DNA of the mutants were isolated, digested with BamHI and ligated into the cloning vector pMD18-T digested with BamHI, then transfered into E. coli DH5α. Positive clones were picked on the plates containing antibiotics（Ampicillin and Kanamicin）. The plasmids of positive clones were digested with BamHI then sequenced for further verified. In total, 17 genes were successfully cloned. This result supported the gene resources for further improving the tolerance of Methylotrophic bacterium to L- senne. |
| Keywords: | Methylobacterium sp. MB200 mutants L-serine tolerance gene clone |
| 本文献已被 维普 万方数据 等数据库收录！ | |