Morphological aspects of neuromuscular junctions and gene expression of nicotinic acetylcholine receptors (nAChRs) in skeletal muscle of rats with heart failure |
| |
Authors: | Paula Aiello Tomé de Souza Selma Maria Michelin Matheus Eduardo Paulino Castan Dijon Henrique Salomé Campos Antônio Carlos Cicogna Robson Francisco Carvalho Maeli Dal-Pai-Silva |
| |
Institution: | 1.Department of Morphology,Institute of Biosciences, UNESP,Botucatu,Brazil;2.Department of Anatomy,Institute of Biosciences, UNESP,Botucatu,Brazil;3.Department of Internal Medicine,UNESP,Botucatu,Brazil |
| |
Abstract: | HF is syndrome initiated by a reduction in cardiac function and it is characterized by the activation of compensatory mechanisms.
Muscular fatigue and dyspnoea are the more common symptoms in HF; these may be due in part to specific skeletal muscle myopathy
characterized by reduced oxidative capacity, a shift from slow fatigue resistant type I to fast less fatigue resistant type
II fibers and downregulation of myogenic regulatory factors (MRFs) gene expression that can regulate gene expression of nicotinic
acetylcholine receptors (nAChRs). In chronic heart failure, skeletal muscle phenotypic changes could influence the maintenance
of the neuromuscular junction morphology and nAChRs gene expression during this syndrome. Two groups of rats were studied:
control (CT) and Heart Failure (HF), induced by a single intraperitoneal injection of monocrotaline (MCT). At the end of the
experiment, HF was evaluated by clinical signs and animals were sacrificed. Soleus (SOL) muscles were removed and processed
for morphological, morphometric and molecular NMJ analyses. Our major finding was an up-regulation in the gene expression
of the alpha1 and epsilon subunits of nAChR and a spot pattern of nAChR in SOL skeletal muscle in this acute monocrotaline
induced HF. Our results suggest a remodeling of nAChR alpha1 and epsilon subunit during heart failure and may provide valuable
information for understanding the skeletal muscle myopathy that occurs during this syndrome. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|