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Indirect photometric ion chromatographic analysis of anions in Haematococcus pluvialis culture media
Authors:Jian-Ping Yuan  Feng Chen
Institution:(1) College of Food Engineering and Biotechnology, South China University of Technology, Guangzhou, 510641, China;(2) Department of Botany, The University of Hong Kong, Hong Kong, China
Abstract:An indirect photometric ion chromatographic method for the simultaneous determination of chloride, nitrate and sulfate ions was developed and applied to the determination of anions, mainly nitrate, in the alga Haematococcus pluvialis culture media. Using phthalic acid/sodium tetraborate aqueous solution as the mobile phase, anions can be detected indirectly by a UV detector. The calibration curves for these anions gave good linearity from 1 to 1000 mgrg ml–1.
Keywords:culture media  determination  Haematococcus pluvialis  inorganic anions  ion chromatography
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