Metabolism of Glutamate in Suspension Cultures of Paul's Scarlet Rose Cells

When specifically labeled glutamate-1-¹⁴C was provided to 4-day-old rose cells, 87.6% of the ¹⁴C in glutamate recovered from protein was in the number 1 carbon atom of the glutamate molecule. It was concluded that newly absorbed glutamate was incorporated directly into protein without any prior metabolism.