Accumulation of orally administered quercetin in brain tissue and its antioxidative effects in rats |
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Authors: | Ishisaka Akari Ichikawa Satomi Sakakibara Hiroyuki Piskula Mariusz K Nakamura Toshiyuki Kato Yoji Ito Mikiko Miyamoto Ken-ichi Tsuji Akira Kawai Yoshichika Terao Junji |
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Affiliation: | a Department of Food Science, Graduate School of Nutrition and Biosciences, University of Tokushima, Tokushima 770-8503, Japanb Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Polandc School of Human Science and Environment, University of Hyogo, Himeji, Japand Department of Molecular Nutrition, Graduate School of Nutrition and Biosciences, University of Tokushima, Tokushima 770-8503, Japane Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Japan |
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Abstract: | Quercetin is widely distributed in vegetables and herbs and has been suggested to act as a neuroprotective agent. Here, we demonstrate that quercetin can accumulate enough to exert biological activity in rat brain tissues. Homogenates of perfused rat brain without detectable hemoglobin contaminants were treated with β-glucuronidase/sulfatase and the released quercetin and its methylated form were analyzed using high-performance liquid chromatography (HPLC) with three different detection methods. Both quercetin and the methylated form were detected in the brain of quercetin-administered rats using HPLC-UV and HPLC with electrochemical detection and were further identified using HPLC-tandem mass spectrometry. Oral administration of quercetin (50 mg/kg body wt) attenuated the increased oxidative stress in the hippocampus and striatum of rats exposed to chronic forced swimming. The possible transport of quercetin derivatives into the brain tissue was reproduced in vitro by using a rat brain capillary endothelial cell line, a model of the blood-brain barrier. These results show that quercetin could be a potent nutrient that can access the brain and protect it from disorders associated with oxidative stress. |
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Keywords: | HPLC, high-performance liquid chromatography UV, ultraviolet Q3GA, quercetin-3-O-β- smallcaps" >d-glucuronide HPLC-ECD, HPLC-electrochemical detection LC-MS/MS, HPLC-tandem mass spectrometry SRM, selected reaction monitoring TBARS, thiobarbituric acid-reactive substances GSH, reduced glutathione BBB, blood-brain barrier |
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