Ultrastructure ofDigitalis lanata tissue cultures |
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Authors: | Britt Berggren Anna B Ohlsson |
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Institution: | (1) Department of Botany, University of Stockholm, Stockholm;(2) Department of Biochemistry and Biotechnology, The Royal Institute of Technology, Stockholm;(3) Palynological Laboratory, Swedish Museum of Natural History, S-104 05 Stockholm, Sweden |
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Abstract: | Summary The anatomy of twoDigitalis lanata tissue culture strains, S-1 and S-2, has been studied. The cardenolide accumulating cell aggregates consisted of highly vacuolated cells with very lobed nuclei in the periphery and a central part of meristematic cells. Sieve tube elements and companion cells and tracheids were observed in some of the cultures. The effect of gibberellic acid (GA3) and SAN 9789 on the ultrastructure of the cultures was most apparent as regards the plastids and the amounts of mitochondria and ER. The content of starch seemed to be highest in the cardenolide accumulating strain S-2 grown in darkness (S-2 D) with or without GA3, but considerable amounts were also found in S-1 grown in darkness (S-1 D) with or without GA3, which did not accumulate cardenolides. The amount of plastoglobuli was increased in S-1 by SAN treatment. It was also higher in S-2 D and S-2 D+GA3 than in S-1 D and S-1 D+GA3; i.e., it was high in tissues with blocked carotene synthesis. Many large plastoglobuli were also observed in apparently degenerating cells. The amount of ER seemed relatively high in cardenolide producing cultures. The amount of mitochondria was highly variable, but no correlation with cardenolide accumulation could be found.Abbreviations D
dictyosome
- ER
endoplasmic reticulum
- M
mitochondrion
- N
nucleus
- P
plastid
- PG
plastoglobule
- S
starch grain
- SC
sieve cell
- V
vacuole
- W
cell wall
- GA3
gibberellic acid
- S-1D
strain S-1 cultured in darkness
- S-1 L
strain S-1 cultured in light
- S-2 D
strain S-2 cultured in darkness
- S-2L8
strain S-2 previously cultured in darkness followed by 8 days in light in the present study
- SAN
SAN 9789 (Norflurazon) 4-chloro-5-(methylamino)-2-( , , ,-trifluoro-m-tolyl)-3(2H)-pyridazinone |
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Keywords: | Cardenolide production Digitalis Gibberellic acid Light Norflurazon Plastids SAN 9789 Tissue cultures |
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