Overexpression of cyclin D1 induces the reprogramming of differentiated epidermal cells into stem cell-like cells |
| |
| Authors: | Along Zhao Leilei Yang Kui Ma Mengli Sun Lei Li Jin Huang |
| |
| Affiliation: | 1. Key Research Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing, P.R. China;2. Tianjin Medical University, Tianjin, PR China;3. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing, P.R. China |
| |
| Abstract: | It has been reported that Wnt/β-catenin is critical for dedifferentiation of differentiated epidermal cells. Cyclin D1 (CCND1) is a β-catenin target gene. In this study, we provide evidence that overexpression of CCND1 induces reprogramming of epidermal cells into stem cell-like cells. After introducing CCND1 gene into differentiated epidermal cells, we found that the large flat-shaped cells with a small nuclear-cytoplasmic ratio changed into small round-shaped cells with a large nuclear-cytoplasmic ratio. The expressions of CK10, β1-integrin, Oct4 and Nanog in CCND1 induced cells were remarkably higher than those in the control group (P < 0.01). In addition, the induced cells exhibited a high colony-forming ability and a long-term proliferative potential. When the induced cells were implanted into a wound of laboratory animal model, the wound healing was accelerated. These results suggested that overexpression of CCND1 induced the reprogramming of differentiated epidermal cells into stem cell-like cells. This study may also offer a new approach to yield epidermal stem cells for wound repair and regeneration. |
| |
| Keywords: | Cyclin D1 dedifferentiation epidermal stem cells reprogramming wound repair |
|
|
