A small phospholipase inhibitory factor released by cultured cell lines |
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Authors: | J N Wood P R Coote J Salmon J Rhodes |
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Institution: | Department of Experimental Immunobiology and Department of Prostaglandin Research, The Wellcome Research Laboratories, Beckenham, Kent, England |
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Abstract: | Cells of the mouse macrophage-like cell line RAW264 release a dialysable inhibitor of phospholipase activity into their culture medium. This inhibitor can be detected in saline solution, Hanks solution and a variety of tissue culture media in the presence or absence of serum. The inhibitor is stable at 4 degrees C, unaffected by trypsin, nucleases, or boiling, and partially extractable with chloroform/methanol. The release of both arachidonic acid and prostaglandins from mouse macrophages or human monocytes is inhibited by this material. A variety of other cell types release the inhibitor, which is effective against stimulation of arachidonic acid release from cultured macrophages by zymosan, serum, immune complexes and the calcium ionophore A23187. |
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