Amylase isozyme polymorphism in maize

Summary Amylase isozyme polymorphisms were shown to exist in certain genetic stocks of Zea mays, using the technique, of starch gel electrophoresis. The earliest period of amylase activity was detected during the second day after germination of seeds. The third day after germination gave the best resolution of amylase activity in the form of distinct zones. Tissues examined from adult plants showed no amylase activity. Several hybrid patterns were of interest, but the most unique situation is the F₁ hybrid AA9xAA2. One of the zones in this hybrid appears to be intermediate to the parental types, suggesting the formation of a hybrid enzyme. In the F₁ hybrid AA7xP51 there are two zones of enzyme activity neither of which is present in the parental strains; this is also suggestive of hybrid enzymes with altered electrophoretic mobilities.Supported by the U.S. Atomic Energy Commission under contract No. AT (11-1) 1338. Portion of this work was done in the Department of Genetics, University of Hawaii, under support from the U.S. National Institutes of Health