Time course study of L-tyrosine aminotransferase induction in rat liver cell lines |
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Authors: | Martine Chessebeuf Michel Fischbach Prudent Padieu |
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Institution: | (1) Faculte de Medecine, Laboratoire de Biochimie Médicate, Dijon, France;(2) Faculte de Medecine, Unite INSERM U 208, Laboratoire de Biochimie Mddicale, 7, blvd Jeanne d'Arc, 21033 Dijon, France |
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Abstract: | The enhancement of L-tyrosine aminotransferase activity by dexamethasone, an exclusive function of the liver, was serially measured at different passages of eight rat liver epithelial cell lines initiated and continuously grown in either a serum-supplemented medium or a serum-free medium. The enzyme basal activity was found to be 5.4 ± 1.8 mU for cell lines in serum and 6.8 ± 3.4 mU for cell lines without serum. Under the influence of dexamethasone (10–6 mol/l for 5 hours) this basal level could be increased up to 2.9 fold in the presence of serum and 2.5 fold in its absence when investigations were carried out at early passages. During the following subcultures the induction ratio gradually declined and scarcely any induction could be detected after the 15th passage for cells grown in serum and after the 25th passage for cell lines grown without serum.Abbreviations SFM
serum-free medium
- SSM
serum-supplemented medium
- TAT
L-tyrosine aminotransferase
M.F. is a recipient of a government scholarship grant from the Grand Duchd de Luxembourg. |
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Keywords: | rat liver epithelial cell cell culture serum tyrosine aminotransferase enzyme induction |
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