Production of 2,3-butanediol by newly isolated Enterobacter cloacae |
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Authors: | B C Saha R J Bothast |
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Institution: | (1) Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA e-mail: sahabc@mail.ncaur.usda.gov Tel.: +1-309-6816276 Fax: +1-309-6816686, US |
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Abstract: | Enterobacter cloacae NRRL B-23289 was isolated from local decaying wood/corn soil samples while screening for microorganisms for conversion of
l-arabinose to fuel ethanol. The major product of fermentation by the bacterium was meso-2,3-butanediol (2,3-BD). In a typical
fermentation, a BD yield of 0.4 g/g arabinose was obtained with a corresponding productivity of 0.63 g/l per hour at an initial
arabinose concentration of 50 g/l. The effects of initial arabinose concentration, temperature, pH, agitation, various monosaccharides,
and multiple sugar mixtures on 2,3-BD production were investigated. BD productivity, yield, and byproduct formation were influenced
significantly within these parameters. The bacterium utilized sugars from acid plus enzyme saccharified corn fiber and produced
BD (0.35 g/g available sugars). It also produced BD from dilute acid pretreated corn fiber by simultaneous saccharification
and fermentation (0.34 g/g theoretical sugars).
Received: 17 December 1998 / Revision received: 9 March 1999 / Accepted: 20 March 1999 |
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