Biomimetic nanofibrous scaffolds: preparation and characterization of chitin/silk fibroin blend nanofibers |
| |
Authors: | Park Ko Eun Jung Sung Youn Lee Seung Jin Min Byung-Moo Park Won Ho |
| |
Institution: | aDepartment of Textile Engineering, Chungnam National University, Daejeon 305–764, South Korea bDepartment of Oral Biochemistry and Craniomaxillofacial Reconstructive Science, Dental Research Institute, and BK21 HLS, Seoul National University College of Dentistry, Seoul 110–749, South Korea cCollege of Pharmacy, Ewha Wowans University, Seoul 120–750, South Korea |
| |
Abstract: | Electrospinning of chitin/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was investigated to fabricate a biomimetic nanostructured scaffolds for tissue engineering. The morphology of the electrospun chitin/SF blend nanofibers was investigated with a field emission scanning electron microscope (FE-SEM). The average diameters of chitin/SF blend fibers decreased from 920 to 340 nm, with the increase of chitin content in blend compositions. The miscibility of chitin/SF blend fibers was examined by solution viscosity measurement. The chitin and SF were immiscible in the as-spun nanofibrous structure. The dimensional stability of chitin/SF blend nanofibers, with or without water vapor after-treatment, was conducted by immersing in water. As-spun SF-rich blend nanofibrous matrices were lost their fibrous structure after the water immersion for 24 h, and then changed into membrane-like structure. On the contrary, nanofibrous structures of water vapor-treated SF-rich blends were almost maintained. To assay the cytocompatibility and cell behavior on the chitin/SF blend nanofibrous scaffolds, cell attachment and spreading of normal human epidermal keratinocyte and fibroblasts seeded on the scaffolds were studied. Our results indicate that chitin/SF blend nanofibrous matrix, particularly the one that contained 75% chitin and 25% SF, could be a potential candidate for tissue engineering scaffolds because it has both biomimetic three-dimensional structure and an excellent cell attachment and spreading for NHEK and NHEF. |
| |
Keywords: | Electrospinning Chitin Silk fibroin Blend nanofiber Matrix Fibroblast |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|