Expression of the complement-binding protein (MP60) of Candida albicans in experimental vaginitis |
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Authors: | Stringaro A |
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Institution: | Laboratories of Ultrastructures, Istituto Superiore di Sanita, Rome, Italy. |
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Abstract: | The expression of the Candida albicans complement-binding C3d protein (MP60) was investigated both in vitro and in vivo by
immunogold labelling and electron microscopy. In vivo expression was determined in a rat vaginitis model. Reactivity of in
vitro-grown cells to an anti-MP60 rabbit serum was associated with both cytoplasmic and cell wall sites. Immunostaining in
the cell wall of both yeast and hyphae was most concentrated in the inner, electron-lucid layer. Immunogold stained preparations
of C. albicans from vaginal smears of infected animals also showed intense localization of the MP60 in the inner cell wall,
plasma membrane. However, immunogold label was also intense at the cell surface in these samples, mostly in the area of close
adherence with the keratinocytes of the vaginal epithelia. These observations indicate that MP60 is expressed both in vitro
and in vivo, but to a different degree in the different cell wall layers.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Candida albicans complement receptor immunoelectron microscopy vaginal infection |
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