| 小牛类表皮生长因子活性肽（c－EGF）的分离纯化及性质 |
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| 引用本文： | 汤国枝,李素芹,张鹤云,李敏意,金以丰. 小牛类表皮生长因子活性肽（c－EGF）的分离纯化及性质[J]. Acta biochimica et biophysica Sinica, 1996, 0(1) |
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| 作者姓名： | 汤国枝 李素芹 张鹤云 李敏意 金以丰 |
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| 作者单位： | 南京大学生物化学系 |
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| 摘 要： | 本文报道小牛颌下腺通过酸抽提、ＣＭ５２、ＤＥ５２柱层析、ＳｅｐｈａｄｅｘＧ７５凝胶过滤及ＨＰＬＣ等步，分离纯化到一个分子量为６ｋＤ，等电点为４．６的小肽。该小肽类似人重组表皮生长因子（ｒｈ—ＥＧＦ）的生物活性，具有刺激ＮＲＫ细胞的增殖及刺激新生小鼠的睁眼、萌牙，并具有增加Ａ４３１细胞膜蛋白质磷酸化的功能。氨基酸组成缺少苯丙氨酸及苏氨酸残基。我们命名它为小牛类ＥＧＦ样活性肽（ｃａｌｆＥＧＦ－ｌｉｋｅａｃｔｉｖｅｐｅｐｔｉｄｅ，简称ｃ－ＥＧＦ）。
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| 关 键 词： | 表皮生长因子；活性肽 |
Purification and Characterization of EGF-like Active Peptide from Calf Submaxillary Gland (c-EGF) |
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| TANG Guo-Zhi, LI Su-Qin, ZHANG He-Ynn,LI Min-Yi and JIN Yi-Feng. Purification and Characterization of EGF-like Active Peptide from Calf Submaxillary Gland (c-EGF)[J]. Acta biochimica et biophysica Sinica, 1996, 0(1) |
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| Authors: | TANG Guo-Zhi LI Su-Qin ZHANG He-Ynn LI Min-Yi JIN Yi-Feng |
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| Abstract: | A pepside with M. of 6kD and pI at 4.6 was purified from calf submaxillary gland by acid extrsction, CM 52 and DE 52 ion exchange chromatography, Sephadex G75 gel filtration and HPLC. Similar to rh-EGF, the peptide haa such biological effects as the simulation of the proliferation of NRK cells and fetal epithelial cells in vitro,the acceleration of incisor eruption and eyelid opening in new born mice and the stimulation of phosphorylation of the membrane protein of A431cells. The amino acid composition of this peptide is similar to that of rh-EGF,with both deficient in phenylalanine and threonine. |
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| Keywords: | Epidermal growth factor Active peptide |
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