High-density genetic mapping for coffee leaf rust resistance |
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Authors: | Valdir Diola Giovani Greigh de Brito Eveline Teixeira Caixeta Eunize Maciel-Zambolim Ney Sussumu Sakiyama Marcelo Ehlers Loureiro |
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Institution: | (1) Departamento de Gen?tica, Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro, Brazil;(2) Departamento de Biologia Vegetal, Universidade Federal de Vi?osa, Vi?osa, Minas Gerias, Brazil;(3) EMBRAPA Algod?o, N?cleo Cerrado, Goi?nia, Goi?s, Brazil;(4) EMBRAPA Caf?/Bioagro, Brasilia, Distrito Federal, Brazil;(5) BIOCAF?/BIOAGRO, Universidade Federal de Vi?osa, Vi?osa, Minas Gerais, Brazil |
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Abstract: | Coffee leaf rust caused by the fungus Hemileia vastatrix causes considerable economic losses for coffee producers. Although agrochemical products can provide sufficient disease control,
the use of resistant cultivars is a safer alternative. This resistance may be constrained by one or a few genetic factors,
mainly those found in material originating from interspecific hybrids. In this study, the genetic analysis of an F
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population consisting of 224 plants derived from a crossing of Híbrido de Timor UFV 427-15 (resistant) with Catuaí Amarelo
IAC 30 (susceptible) showed that a dominant gene confers the resistance of coffee to race II of H. vastatrix. From a genetic map saturated with 25 amplified fragment length polymorphism (AFLP) markers linked to the resistance gene,
we developed a high-density genetic map with six sequence-characterized amplified region (SCAR) markers delimiting a chromosomal
region of 9.45 cM and flanking the dominant gene at 0.7 and 0.9 cM. This is the first saturated and high-density genetic map
obtained from this region containing the resistance gene. The results of this study are of great importance for the introduction
of molecular markers for marker-assisted selection; they will also facilitate studies related to the cloning, structure, and
function of race-specific genes involved in the resistance of coffee trees to H. vastatrix. |
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