Bioethanol production from tension and opposite wood of <Emphasis Type="Italic">Eucalyptus globulus</Emphasis> using organosolv pretreatment and simultaneous saccharification and fermentation |
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Authors: | Claudio Muñoz Jaime Baeza Juanita Freer Regis Teixeira Mendonça |
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Institution: | 1.Centro de Biotecnología, Universidad de Concepción,Concepción,Chile;2.Facultad de Ciencias Químicas,Universidad de Concepción,Concepción,Chile;3.Facultad de Ciencias Forestales,Universidad de Concepción,Concepción,Chile |
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Abstract: | During tree growth, hardwoods can initiate the formation of tension wood, which is a strongly stressed wood on the upper side
of the stem and branches. In Eucalyptus globulus, tension wood presents wider and thicker cell walls with low lignin, similar glucan and high xylan content, as compared to
opposite wood. In this work, tension and opposite wood of E. globulus trees were separated and evaluated for the production of bioethanol using ethanol/water delignification as pretreatment followed
by simultaneous saccharification and fermentation (SSF). Low residual lignin and high glucan retention was obtained in organosolv
pulps of tension wood as compared to pulps from opposite wood at the same H-factor of reaction. The faster delignification
was associated with the low lignin content in tension wood, which was 15% lower than in opposite wood. Organosolv pulps obtained
at low and high H-factor (3,900 and 12,500, respectively) were saccharified by cellulases resulting in glucan-to-glucose yields
up to 69 and 77%, respectively. SSF of the pulps resulted in bioethanol yields up to 35 g/l that corresponded to 85–95% of
the maximum theoretical yield on wood basis, considering 51% the yield of glucose to ethanol conversion in fermentation, which
could be considered a very satisfactory result compared to previous studies on the conversion of organosolv pulps from hardwoods
to bioethanol. Both tension and opposite wood of E. globulus were suitable raw materials for organosolv pretreatment and bioethanol production with high conversion yields. |
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