Atg8 lipidation is coordinated in a PtdIns3P-dependent manner by the PROPPIN Atg21 |
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Authors: | Roswitha Krick |
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Affiliation: | Institute of Cellular Biochemistry, University Medicine Goettingen, Georg-August-University, G?ttingen, Germany |
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Abstract: | In Saccharomyces cerevisiae Atg8 coupled to phosphatidylethanolamine is a key component of autophagosome biogenesis. Atg21 binds via 2 sites at the circumference of its β-propeller to PtdIns3P at the phagophore assembly site (PAS). It recruits and arranges both Atg8 and Atg16, which is part of the E3-like ligase complex Atg12–Atg5-Atg16. Binding of Atg8 to Atg21 requires the FK-motif within the N-terminal-helical domain of Atg8 and D146 at the top of the Atg21 β-propeller. Atg16 binds via D101 and E102 within its coiled-coil domain to Atg21. |
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Keywords: | Atg8 lipidation Atg16 Atg21 PROPPIN PtdIns3P |
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