Disruption of a filamentous fungal organism (N. sitophila) using a bead mill of novel design I. General disruption characteristics |
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Authors: | Clark V. Baldwin Murray Moo-Young |
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Affiliation: | (1) Department of Chemical Engineering, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada |
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Abstract: | The disruption of a typical filamentous fungus, a native strain of Neurospora sitophila, was studied using a glass bead mill of novel design (the Sulzer Annu Mill 01). Cell concentration (in the range of 2.5–5 g dry weight/L) had little influence on the disruption attained. Disruption increased with increasing rotor speed (1000 –4000 r.p.m.) and number of passes (up to six passes) through the Annu Mill. Disruption was observed to follow traditional first-order kinetics for bead mills possessing predominantly plug flow characteristics. It was concluded that in general the Annu Mill would be applicable for the disruption of filamentous organisms.Nomenclature CP aqueous-phase soluble protein concentration of disrupted sample (g/mL) - CP,MAX aqueous-phase soluble protein concentration of a completely disrupted sample (g/mL) - CPO aqueous-phase soluble protein concentration of undisrupted sample (g/mL) - N number of passes though the bead mill (–) - R total fraction of cells disrupted (–)Greek Letters C internal moisture volume fraction of undisrupted cells (–) - L aqueous phase volume fraction of disrupted cell suspension (–) - LO aqueous phase volume fraction of undisrupted cell suspension (–) - L,MAX aqueous phase volume fraction at complete disruption (R=1) (–) - fluid density (kg/m3) - C density of the microorganism (kg/m3) - L density of the suspending aqueous phase (kg/m3) - suspension batch residence time in the Annu Mill 01 (min.)Abbreviations DW dry weight |
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