| 酶法提取玉米胚中总黄酮的研究 |
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| 引用本文： | 张丽丽,肖旭平,李秉超.酶法提取玉米胚中总黄酮的研究[J].氨基酸和生物资源,2007,29(1):80-82. |
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| 作者姓名： | 张丽丽 肖旭平 李秉超 |
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| 作者单位： | 沈阳农业大学生物技术学院,辽宁沈阳,110161;沈阳军区总院北陵临床部,辽宁沈阳,110031;沈阳农业大学基础部,辽宁沈阳,110161 |
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| 摘 要： | 尝试使用α-淀粉酶进行糖苷转移提取玉米中总黄酮,同时对α-淀粉酶的固定化进行研究。对α-淀粉酶固定化的研究表明:以壳聚糖为载体,用体积分数4%戊二醛进行交联,加酶量为3 g.L-1条件下可以获得最佳的固定化效果;与游离酶相比,其最适作用温度范围、pH值范围均比游离酶范围宽;固定化酶的热稳定性优于游离酶,且具有良好的操作稳定性。本试验使用游离酶法、固定化酶法提取玉米中总黄酮,得率分别为2.82%、2.46%。
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| 关 键 词： | 玉米胚 总黄酮 α-淀粉酶 酶的固定化 |
| 文章编号： | 1006-8376(2007)01-0080-03 |
| 修稿时间： | 10 16 2006 12:00AM |
Study on Enzyme - Based Extraction of the Total Flavonoid from Maize Embryo |
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| ZHANG Li - li, et al.Study on Enzyme - Based Extraction of the Total Flavonoid from Maize Embryo[J].Amino Acids & Biotic Resources,2007,29(1):80-82. |
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| Authors: | ZHANG Li - li |
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| Institution: | College of Biotechnology, Shenyang Agricultural University, Shenyang 110161, China |
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| Abstract: | The immobilization of α-amylase was studied.α-amylase was immobilized on chitosan with glutaraldehyde by cross-linking reaction.The immobilization conditions and partial properties of immobilized enzyme were investigated.The best efficiency of immobilization could be obtained under the conditions that the concentration of glutaraldehyde was 4% and the adding amount of αamylase was 3mg/ml.Compared with the free enzyme,it showed a wider range of reaction temperature and PH value.The thermostability of immobilized α-amylase was better than native enzyme.And it had a higher operational stability.The extraction yields were 2.82% and 2.46% respectively. |
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| Keywords: | maize embryo flavonoids α-amylase immobilization of α-amylase |
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