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| Folin-ciocalteu比色法测桑叶中多酚含量 | |
| 引用本文： | 张军,李江遐,陆翠珍,宛晓春,刘朝良.Folin-ciocalteu比色法测桑叶中多酚含量[J].激光生物学报,2012,21(2):131-135. |
| 作者姓名： | 张军 李江遐 陆翠珍 宛晓春 刘朝良 |
| 作者单位： | 1. 安徽农业大学生命科学学院,安徽合肥,230036 2. 安徽农业大学资源环境学院,安徽合肥,230036 3. 安徽农业大学生物技术中心,安徽合肥,230036 4. 安徽农业大学农业部茶叶生物化学与生物技术重点实验室,安徽合肥,230036 |
| 摘 要： | 采用以没食子酸为标准,研究了Folin-ciocalteu比色法测定桑叶中多酚含量的适宜条件。结果表明,桑叶提取液在Folin-ciocalteu试剂2.0 mL、20%Na2CO3溶液5.0 mL、反应温度30℃、反应时间2 h的条件下,测定其760 nm处的吸光值,多酚浓度在10.0～100.0 mg/L范围内与吸光值呈良好的线性关系;稳定性、精密度、重现性和回收率实验的相对标准偏差为0.2969%～2.502%。该法是一种简便、快速、准确测定桑叶多酚含量的可靠方法。 |
| 关 键 词： | 桑叶 多酚 Folin-ciocalteu比色法 |
Determination of the Total Content of Polyphenols in the Leaf of Mulbery by Folin-ciocalteu Colorimetry |
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| ZHANG Jun , LI Jiangxia , LU Cuizhen , WAN Xiaochun , LIU Chaoliang.Determination of the Total Content of Polyphenols in the Leaf of Mulbery by Folin-ciocalteu Colorimetry[J].ACTA Laser Biology Sinica,2012,21(2):131-135. | |
| Authors: | ZHANG Jun LI Jiangxia LU Cuizhen WAN Xiaochun LIU Chaoliang |
| Institution: | 1 (1. School of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, China; 2. School of Resource and Environment, Anhui Agricultural University, Hefei 230036, Anhui, China ; 3. Key Laboratory of Tea Biochemistry＆Biotechnology, Ministry of Agriculture, Hefei 230036, Anhui, China; 4. Biotechnology Centre, Anhui Agricultural University, Hefei 230036, Anhui, China) |
| Abstract: | A quantitative method for the determination of the total content of polyphenols in the leaf of mulberry was studied by using Folin-eiocalteu colorimetry with gallic acid as standard. The results showed that the total polyphenol contents of the leaf of mulberry could be well calculated according to their colorimetric absorption at 760 nm by applying Folin-ciocalteu reagent 2.0 mL and 20% Na2CO3 5.0 mL at 30℃for 2 h, and the linear range of standard curve was 10.0- 100.0 mg/L. Stability, precision, repetition and recovery rate were in the range of 0. 2969% -2. 502% by RSD. Therefore, Folin-ciocaheu colorimetry is convenient, fast, and accurate to determine total polyphenols of the leaf of mulbery. |
| Keywords: | the leaf of mulberry polyphenol Folin-eiocalteu colorimetry |
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