| 大鼠心肌发育相关基因在心肌梗死后的表达变化 |
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| 引用本文： | 姜涛,王春梅,来仪,贾竹青,刘永刚,马康涛,李刚,周春燕.大鼠心肌发育相关基因在心肌梗死后的表达变化[J].中国生物化学与分子生物学报,2004,20(6):833-837. |
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| 作者姓名： | 姜涛 王春梅 来仪 贾竹青 刘永刚 马康涛 李刚 周春燕 |
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| 作者单位： | 1. 北京大学基础医学院生物化学与分子生物学系,北京,100083
2. 北京大学第三医院心内科,北京,100083 |
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| 基金项目： | 国家高技术研究发展计划 (863计划 )基金 (2 0 0 1AA2 160 3 13 )，北京市科技计划重大项目基金 (H0 2 0 2 2 0 0 10 490 )，教育部 2 11工程和北京大学985基金资助项目~~ |
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| 摘 要： | 探讨大鼠急性心肌梗死后与心肌发育相关基因的表达变化 .通过结扎大鼠冠状动脉左前降支建立急性心肌梗死模型 ,利用逆转录聚合酶链式反应 (RT PCR)和Northern印迹技术分别检测GATA4、Nkx2 5BMP2和Wnt11基因在心肌梗死后不同时间的表达变化趋势 .结果显示 ,在正常心肌中 ,与心肌发育相关的基因GATA4、Nkx2 5和BMP2有较强的表达 ,而Wnt11基础表达较低 .心肌梗死后 ,GATA4、Nkx2 5和BMP2表达下降 ,7d后有所恢复 ;Wnt11的表达在心肌梗死后呈上升趋势 ,至梗死 7d达到高峰 .结果提示 ,心肌发育相关基因的表达在心肌梗死后有明显改变 ,并发现启动心肌发育的关键信号分子———Wnt11基因表达上调 ,为心肌损伤后细胞分化机制的研究提供线索
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| 关 键 词： | 心肌梗死 GATA4 Nkx25 BMP2 Wnt11 |
| 收稿时间： | 2004-12-20 |
| 修稿时间： | 2004年1月6日 |
Cardiogenesis-related Gene Expression Changes after Myocardial Infarction in Rats |
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| JIANG Tao ,WANG Chun-mei ,LAI Yi ,JIA Zhu-qing ,LIU Yong-gang ,MA Kang-tao ,LI Gang ,ZHOU Chun-yan.Cardiogenesis-related Gene Expression Changes after Myocardial Infarction in Rats[J].Chinese Journal of Biochemistry and Molecular Biology,2004,20(6):833-837. |
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| Authors: | JIANG Tao WANG Chun-mei LAI Yi JIA Zhu-qing LIU Yong-gang MA Kang-tao LI Gang ZHOU Chun-yan |
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| Institution: | ( 1)Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences,Peking University,Beijing 100083,China; 2)Department of Cardiology, Peking University Third Hospital, Beijing 100083,China |
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| Abstract: | The expression profiles of GATA4, Nkx2.5, BMP2 and Wnt11 after myocardial infarction (MI) in rats were investigated by RT-PCR and Northern-blot analysis. GATA4, Nkx2.5 and BMP2 were highly expressed in normal rats. The basal expression of Wnt11 was low. After MI, the expressions of Nkx2.5, GATA4 and BMP2 were down-regulated markedly and recovered gradually after seven days. In contrast, the expression of Wnt11, which initiates heart development, was up-regulated, and reached the peak on day-seven after MI. The results indicated that heart developmental-related genes had different responses to the MI. The study provided an insight into cell differentiation under myocardial injury conditions. |
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| Keywords: | myocardial infarction GATA4 Nkx2 5 BMP2 Wnt11 |
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