Antibodies to the most tightly bound proteins in eukaryotic DNA : Formation of immuno-complexes with ‘nuclear matrix’ components |
| |
Authors: | Dieter Werner, Shi Chanpu, Michael Mü ller, Eberhard Spiess,Ulrich Plagens |
| |
Affiliation: | Institute of Cell and Tumor Biology, German Cancer Research Center, D-6900 Heidelberg, Germany |
| |
Abstract: | Chromosomal DNA is associated with polypeptides covalently bound to internal DNA ends. Since these polypeptides can only be released from chromosomal DNA by enzymes or other agents hydrolysing phosphodiester bonds they were termed 'the most tightly bound' (MTB) polypeptides in DNA. Antibodies developed against the MTB polypeptides are shown to form immunocomplexes with major 'nuclear matrix' polypeptides as well as with polypeptides which are still associated with 'nuclear matrix' DNA isolated by means of SDS/proteinase K and phenol. Immuno-complex formation is revealed by immunoblotting and by indirect immunofluorescence. Thus, since MTB polypeptides, major 'nuclear matrix' polypeptides and 'nuclear matrix' DNA-associated polypeptides share common antigenic sites they can be considered to be identical or at least closely related. This suggests that a fraction of distinct 'nuclear matrix' polypeptides is either transiently or permanently linked to DNA by covalent bonds. Consistently, isolated eukaryotic 'bulk' DNA is inevitably associated with residual 'nuclear matrix' polypeptides. |
| |
Keywords: | To whom offprint requests should be sent. |
本文献已被 ScienceDirect 等数据库收录! |
|