A rapid coupling protocol for the synthesis of peptide nucleic acids |
| |
Authors: | Vearing Christopher J Fecondo John V |
| |
Institution: | (1) School of Engineering and Science, Swinburne University of Technology, Hawthorn, Victoria, Australia;(2) Department of Biotechnology and Environmental Biology, RMIT University, Bundoora, Victoria, Australia |
| |
Abstract: | Summary With the current interest in anti-sense and anti-gene technologies, an efficient, fast and less toxic synthesis protocol would
be advantageous for the oligomerisation of Peptide Nucleic Acids (PNA). Most of the methods currently in use for thet-Boc synthesis of PNA's use TFA/m-cresol, pyridine, piperidine and capping reagents. In this work, a rapid synthesis protocol has been adapted from an earlier
published peptide synthesis method allowing a reduction in cycle time from around 30 min down to 16 min. By utilising quantitative
deprotection with 100% TFA, a coupling time of 10 min and a four-fold excess of monomer, this synthesis protocol has been
used to synthesise a number of PNA's incorporating all four nucleotides of varying sequence, up to 17 residues in length. |
| |
Keywords: | anti-sense/anti-gene oligonucleotide peptide nucleic acid PNA SPPS |
本文献已被 SpringerLink 等数据库收录! |
|