Fine mapping of the clubroot resistance gene, Crr3, in Brassica rapa |
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| Authors: | M Saito N Kubo S Matsumoto K Suwabe M Tsukada M Hirai |
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| Institution: | (1) Graduate School of Agriculture, Kyoto Prefectural University, Kyoto Prefectural Institute of Agricultural Biotechnology, 74 Oji, Kitainayazuma, Seika, Soraku, Kyoto 619-0244, Japan;(2) Present address: Nanto Seed Co., Ltd, Kashihara, Nara 634-007, Japan;(3) Department of Physiology and Quality Science, National Institute of Vegetable and Tea Science (NIVTS), National Agriculture and Food Research Organization, Tsubaka Tsu, Mie, 514-2392, Japan;(4) Present address: Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK;(5) Nagano Vegetable and Ornamental Crops Experiment Station, Matsushiro, Nagano 381-1211, Japan;(6) Present address: JA Nagano, National Federation of Agricultural Co-operative Associations (Zen-noh), Minami-Nagano, Nagano 380-8614, Japan |
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| Abstract: | A linkage map of Chinese cabbage (Brassica rapa) was constructed to localize the clubroot resistance (CR) gene, Crr3. Quantitative trait loci analysis using an F3 population revealed a sharp peak in the logarithm of odds score around the sequence-tagged site (STS) marker, OPC11-2S. Therefore, this region contained Crr3. Nucleotide sequences of OPC11-2S and its proximal markers showed homology to sequences in the top arm of Arabidopsis chromosome 3, suggesting a synteny between the two species. For fine mapping of Crr3, a number of STS markers were developed based on genomic information from Arabidopsis. We obtained polymorphisms in 23 Arabidopsis-derived STS markers, 11 of which were closely linked to Crr3. The precise position of Crr3 was determined using a population of 888 F2 plants. Eighty plants showing recombination around Crr3 locus were selected and used for the mapping. A fine map of 4.74 cM was obtained, in which two markers (BrSTS-41 and BrSTS-44) and three markers (OPC11-2S, BrSTS-54 and BrSTS-61) were cosegregated. Marker genotypes of the 21 selected F2 families and CR tests of their progenies strongly suggested that the Crr3 gene is located in a 0.35 cM segment between the two markers, BrSTS-33 and BrSTS-78. |
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