The sorcin-annexin VII calcium-dependent interaction requires the sorcin N-terminal domain |
| |
Authors: | Verzili D Zamparelli C Mattei B Noegel A A Chiancone E |
| |
Institution: | CNR Center of Molecular Biology, Department of Biochemical Sciences 'A. Rossi Fanelli', University of Rome 'La Sapienza', P.le A. Moro 5, 00185, Rome, Italy. |
| |
Abstract: | Dolichyl monophosphate (Dol-P) has been found to induce apoptosis in human leukemia U937 cells. During this apoptotic execution, the increase of plasma membrane fluidity (5–20 min), caspase-3-like protease activation (2–4 h), chromatin condensation and DNA ladder formation (3–4 h) were observed successively. Here, we report that reduction in mitochondrial transmembrane potential and translocation of apoptosis-inducing factor (AIF) are early events (1–3 h) in the apoptotic process induced by Dol-P in U937 cells. The AIF was concentrated around nuclei and partly translocated to the nuclei, which was confirmed by immunocytochemistry using specific anti-AIF antibody. Both caspase-8 and caspase-3 inhibitors blocked only DNA fragmentation but not mitochondrial processes, AIF migration and chromatin condensation. These results indicate that mitochondrial changes are an early step in the apoptosis induced by Dol-P and AIF is one of the important factors which induce chromatin condensation in nuclei. |
| |
Keywords: | Apoptosis-inducing factor Dolichyl monophosphate Apoptosis Mitochondrion Caspase |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|