鲍曼不动杆菌对碳青霉烯类抗生素的耐药性分析

目的了解鲍曼不动杆菌的耐药情况,并检测耐碳青霉烯类鲍曼不动杆菌的耐药基因,为指导临床合理用药、控制院内感染提供依据。方法利用K-B法检测45株鲍曼不动杆菌临床分离株的耐药情况,通过改良Hodge试验、Carba NP试验和EDTA协同试验对多重耐药鲍曼不动杆菌的碳青霉烯酶进行表型检测,并采用PCR技术检测鲍曼不动杆菌携带OXA-23和NDM-1型耐药基因的情况。结果 45株鲍曼不动杆菌临床分离株中共筛出42株多重耐药菌株;利用改良Hodge试验和Carba NP试验检出36株碳青霉烯酶阳性菌株;采用PCR扩增出OXA-23,未扩增出NDM-1。结论鲍曼不动杆菌耐药情况严重,且耐药基因OXA-23携带率高,治疗时应根据药敏试验结果合理用药。

The resistance of Acinetobacter baumannii to carbapenem antibiotics

Abstract: Objective To learn the drug resistance of Acinetobacter baumannii and detect the resistant gene of carbapenem-resistant Acinetobacter baumannii, so as to provide theoretical basis for clinical rational use of drugs and control of nosocomial infections. Methods The drug resistance of 45 strains of Acinetobacter baumannii clinical isolates were detected by using K-B method. The phenotype of carbapenemase was analyzed through modified Hodge test, Carba NP test and EDTA co-trial. The OXA-23 and NDM-1 genes in Acinetobacter baumannii were detected with PCR. Results A total of 42 multidrug-resistant strains were detected. 36 strains of carbapenemase positive strains were detected by using modified Hodge test and Carba NP test. OXA-23 gene was amplified by using PCR while no NDM-1 gene was found. Conclusion The drug resistance of Acinetobacter baumannii is very high, and most of the strains carry OXA-23 gene. Antibacterial drugs should be used reasonably based on the results of drug susceptibility test.