布拉酵母菌对大鼠非酒精性脂肪性肝炎的疗效

目的 探讨布拉酵母菌在高脂饮食诱导的非酒精性脂肪性肝炎（NASH）大鼠模型中的疗效及机制。方法 36只雄性SD大鼠随机分为3组：A组（普通饲料组）、B组（高脂饲料组）和C组（高脂饲料+布拉酵母菌组）。实验第6周起，C组给予布拉酵母菌散10 mg/kg灌胃。第16周末进行肝脏病理分析和血清空腹ALT、AST、脂多糖（LPS）、肿瘤坏死因子-α（TNF-α）、脂联素以及丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性测定；检测粪便中双歧杆菌、乳杆菌、肠球菌和大肠埃希菌数量，分析菌群与LPS相关性。结果 与A组相比，模型B组肝脏脂肪变性程度和肝脏炎症显著增加，粪便菌群发生紊乱；与B组相比，C组脂肪变性及炎症程度积分显著减轻，血清ALT、AST、LPS、TNF-α及MDA显著下降，脂联素水平和SOD活性增高，大鼠粪便中乳杆菌、双歧杆菌和肠球菌数量明显增高，大肠埃希菌显著降低；乳杆菌、双歧杆菌和肠球菌与LPS负相关，大肠埃希菌与LPS正相关。结论 布拉酵母菌可通过调整肠道菌群结构，减少炎性因子释放，拮抗脂质过氧化，改善肝脏脂肪变性及炎症。

Research of anti-inflammatory effect of Saccharomyces boulardii of NASH Rat model

Objective To investigate the efficacy of Saccharomyces boulardii on experimental NASH rats and explore its possible mechanisms. Methods Thirty six SD rats were randomly divided into three groups: group A fed a standard diet, group B fed high-fat diet and group C fed a high fat diet for 6 weeks followed by 10-week gavage of 10 mg/kg Saccharomyces boulardii powder. Hepatic histological change was observed by hematoxylin and eosin stain. Serum fasting ALT, AST, LPS, tumor necrosis factor-α (TNF-α), adiponectin and the malondialdehyde (MDA) and superoxide dismutase (SOD) activity of hepatic tissue were measured at the end of the 16th week. Fecal Bifidobacteria, Lactobacillus, Enterococcus and Escherichia coli were detected, and analysing the relevance between LPS and microbiota. Results At the end of the 16th week, the liver index, compare with group A, steatosis and inflammation of hepatic lobule increased significantly and fecal microbiota dysbiosis in group B. Compared with group B, steatosis and inflammation of hepatic lobule decreased significantly in group C, which were accompanied by decreased significantly of level of serum ALT, AST, LPS and TNF-α and hepatic MDA, and elevated of adiponectin and SOD activity. Microbiota analysis showed the numbers of Lactobacilli, Bifidobacteria and Enterococci in group B were lower than in group C, while the number of E. coli decreased significantly. Lactobacilli, Bifidobacteria and Enterococci had the negative relevance with LPS, while E. coli showed the positive relevance. Conclusion Saccharomyces boulardii can ameliorate inflammation of liver tissue in experimental NASH. The possible mechanisms involve modulating intestinal microbiota, decreasing the production of inflammatory factor, and preventing lipid peroxidation.