In vitro plant regeneration from leaf callus of Grindelia robusta Nutt |
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Authors: | Daniele Fraternale Laura Giamperi Anahi Bucchini Donata Ricci |
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Affiliation: | 1. Istituto di Botanica e Orto Botanico , Urbino (PU), Italy d.fraternale@uniurb.it;3. Istituto di Botanica e Orto Botanico , Urbino (PU), Italy |
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Abstract: | Abstract A regeneration protocol from leaf explants of Grindelia robusta Nutt. was developed. The combination of 0.5 mg l?1 IBA plus 0.5 mg l?1 or 1 mg l?1 BA added to Murashige-Skoog (MS) medium resulted in the best callus induction frequency; the combination of 0.4 or 0.9 mg l?1 BA plus 1.2 mg l?1 GA3 resulted in the best shoot regeneration. Rooting was successful on MS medium supplemented with 0.5 mg l?1 IBA. Hardening of G. robusta plants was accomplished in 30 days with 85% survival rate. |
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Keywords: | Callus culture Grindelia robusta growth regulators plant regeneration |
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