Genome Sequence of the Bacterium Streptomyces davawensis JCM 4913 and Heterologous Production of the Unique Antibiotic Roseoflavin |
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| Authors: | Frank Jankowitsch Julia Schwarz Christian Rückert Bertolt Gust Rafael Szczepanowski Jochen Blom Stefan Pelzer J?rn Kalinowski Matthias Mack |
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| Institution: | aDepartment of Biotechnology, Institute for Technical Microbiology, Mannheim University of Applied Sciences, Mannheim, Germany;bInstitute for Genome Research and Systems Biology, Center for Biotechnology, Bielefeld University, Bielefeld, Germany;cPharmaceutical Biology, Pharmaceutical Institute, Eberhard Karls-Universität Tübingen, Tübingen, Germany;dB.R.A.I.N. AG, Zwingenberg, Germany |
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| Abstract: | Streptomyces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B2) analog. Here, we report the 9,466,619-bp linear chromosome of S. davawensis JCM 4913 and a 89,331-bp linear plasmid. The sequence has an average G+C content of 70.58% and contains six rRNA operons (16S-23S-5S) and 69 tRNA genes. The 8,616 predicted protein-coding sequences include 32 clusters coding for secondary metabolites, several of which are unique to S. davawensis. The chromosome contains long terminal inverted repeats of 33,255 bp each and atypical telomeres. Sequence analysis with regard to riboflavin biosynthesis revealed three different patterns of gene organization in Streptomyces species. Heterologous expression of a set of genes present on a subgenomic fragment of S. davawensis resulted in the production of roseoflavin by the host Streptomyces coelicolor M1152. Phylogenetic analysis revealed that S. davawensis is a close relative of Streptomyces cinnabarinus, and much to our surprise, we found that the latter bacterium is a roseoflavin producer as well. |
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