Up-regulated miR-145 Expression Inhibits Porcine Preadipocytes Differentiation by Targeting IRS1 |
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| Authors: | Yunxue Guo Yaosheng Chen Yun Zhang Yue Zhang Luxi Chen Delin Mo |
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| Institution: | 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006 P. R. China;;2. Guangdong Province Engineering and technology R&D centers for pig breeding, Sun Yat-sen University, Guangzhou, Guangdong 510006 P. R. China. |
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| Abstract: | Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (P<0.05). Furthermore, up-regulation of miR-145 expression repressed induction of mRNA levels of adipogenic markers, such as CCAAT/enhancer-binding protein α (C/EBPα), and peroxisome proliferator-activated receptor γ2 (PPARγ2). These effects caused by miR-145 overexpression were mediated by Insulin receptor substrate 1 (IRS1) as a mechanism. These data suggested that induced miR-145 expression during differentiation could inhibit adipogenesis by targeting IRS1, and miR-145 may be novel agent for adipose tissue engineering. |
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| Keywords: | miR-145 IRS1 inhibit dedifferentiated fat cells adipogenesis |
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