Gateway Role for rRNA Precursors in Ribosome Assembly |
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| Authors: | Nancy S Gutgsell Chaitanya Jain |
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| Institution: | Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA |
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| Abstract: | In Escherichia coli, rRNAs are initially transcribed with precursor sequences, which are subsequently removed through processing reactions. To investigate the role of precursor sequences, we analyzed ribosome assembly in strains containing mutations in the processing RNases. We observed that defects in 23S rRNA processing resulted in an accumulation of ribosomal subunits and caused a significant delay in ribosome assembly. These observations suggest that precursor residues in 23S rRNA control ribosome assembly and could be serving a regulatory role to couple ribosome assembly to rRNA processing. The possible mechanisms through which rRNA processing and ribosome assembly could be linked are discussed. |
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