Influence of drying methods over in vitro antitumoral effects of exopolysaccharides produced by Agaricus blazei LPB 03 on submerged fermentation |
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Authors: | M B A Fernandes S Habu M A de Lima V Thomaz-Soccol C R Soccol |
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Institution: | (1) Bioprocess Engineering and Biotechnology Division, Federal University of Paran?, UFPR, Post Office Box 19011, Curitiba, PR, 81531-990, Brazil; |
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Abstract: | Agaricus blazei is a mushroom that belongs to the Brazilian biodiversity and is considered as an important producer of bioactive compounds
beneficial to human health. Studies have demonstrated that these compounds present immuno-modulatory, antioxidant and antitumor
properties. In order to compare the most used method for fungal polysaccharide drying, lyophilization with other industrial-scale
methods, the aim of this work was to submit A. blazei LPB 03 polysaccharide extracts to vaucum, spray and freeze drying, and evaluate the maintenance of its antitumoral effects in vitro.
Exopolysaccharides produced by A. blazei LPB 03 on submerged fermentation were extracted with ethanol and submitted to drying processes. The efficiency represents the water
content that was removed during the drying process. The resultant dried products showed water content around 3% and water
activity less than 0.380, preventing therefore the growth of microorganisms and reactions of chemical degradation. Exopolysaccharide
extracts dried by vacuum and spray dryer did not showed any significant cytotoxic effect on cell viability of Wistar mice
macrophages. Content of total sugars and protein decrease after drying, nevertheless, 20 mg/ml of exopolysaccharides dried
by spray dryer reached 33% of inhibition rate over Ehrlich tumor cells in vitro. |
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