Suppressor of cytokine signalling protein SOCS3 expression is increased at sites of acute and chronic inflammation |
| |
Authors: | Gemma E White Andrew Cotterill Mark R Addley Elizabeth J Soilleux David R Greaves |
| |
Institution: | (1) Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK;(2) Department of Cellular Pathology, John Radcliffe Hospital, Oxford, OX3 9DU, UK;(3) Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford, OX3 9DU, UK; |
| |
Abstract: | Treatment of cells with cytokines and growth factors leads to the synthesis of Suppressor of Cytokine Signalling (SOCS) proteins
that act as potent negative regulators of signalling via the Jak/STAT pathway. We used immunohistochemistry to identify cells
and pathologies where SOCS3 expression might influence acute and chronic inflammatory responses in human tissues. Epitope
and GFP tagged SOCS3 fusion proteins were localised predominantly in the nucleus of transfected cells and a validated anti
SOCS3 antiserum revealed the expression of SOCS3 in the nucleus and cytoplasm of macrophages, endothelial and epithelial cells
in a wide range of normal tissues in tissue microarrays (n = 31 different tissues). Nuclear SOCS3 was only seen in cells expressing a high level of the protein. Comparative immunostaining
of acute, chronically and granulomatously inflamed human tissues revealed higher levels of nuclear and cytoplasmic SOCS3 expression
in inflamed than in corresponding normal tissues, particularly in recruited leukocyte populations, but also in epithelia.
The staining appeared more intense, suggesting higher expression levels, in areas where inflammation was more acute, consistent
with the time course of SOCS3 induction described in vitro. Expression of SOCS3 protein by leucocytes and other cell types
in tissue sections could be a useful marker of cells undergoing acute or chronic stimulation by cytokines in vivo. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|