Fluorography--limitations on its use for the quantitative detection of 3H- and 14C-labeled proteins in polyacrylamide gels |
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| Authors: | C R Harding I R Scott |
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| Institution: | 1. Department of Biochemistry, University of Washington, Seattle, Washington 98195 USA;2. Howard Hughes Medical Institute Laboratory, University of Washington, Seattle, Washington 98195 USA |
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| Abstract: | The suitability of fluorography for the detection of 3H- and 14C-labeled proteins on polyacrylamide gradient gels has been investigated. It was found that the absorbance of the fluorographic film image produced by a given level of radioactivity decreased as the acrylamide concentration in the gel increased. The use of Coomassie brilliant blue protein dyes to stain the gel prior to fluorography reduced the absorbance of the fluorographic image. It is concluded that quantitative fluorography can only be applied to unstained gels of a uniform acrylamide concentration. |
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| Keywords: | SDS sodium dodecyl sulfate PPO 2 5-diphenyl-oxazole BSA bovine serum albumin |
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