Determination of genetic variability among the isolates of <Emphasis Type="Italic">Metarhizium anisopliae</Emphasis> var. <Emphasis Type="Italic">anisopliae</Emphasis> from different geographical origins |
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Authors: | Shoaib Freed Feng-Liang Jin Shun-Xiang Ren |
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Institution: | (1) Engineering Research Center for Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, 510642, People’s Republic of China |
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Abstract: | The entomopathogenic fungus Metarhizium anisopliae is currently used as an efficient biological control agent against different insects. The prevalence and genetic variability
of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae in Asian countries (China, Laos, Singapore and South Korea) and a European country (The Netherlands) was examined. The fungus
was found to be widespread in agricultural and forest soils throughout China especially in the south and south western regions,
with a maximum recovery percentage of 81.6, while in Laos it was found to be abundant in the forest soils only, the soil of
Netherlands also seemed to be an excellent source of entomopathogens with a significant recovery of insect pathogenic fungi.
Simple sequence repeats (SSR) and ITS-rDNA sequence data showed that the isolates are closely linked to each other. The gene
diversity (He) and polymorphic information content values of sixty two isolates showed mean values of 0.37 and 0.63. The majority
of the isolates belonged to one of the closely related genotypes and these were found to be dominant in the agricultural as
well as forest ecosystem. Genetic distances among the isolates according to locations and different sources showed minimal
variation ranging from 0.23 to 0.34%, with maximum genetic distance of 0.34% among the isolates from Laos and The Netherlands.
The reason for the limited variation is uncertain, however even the similarity index among the isolates can endow with sufficient
knowledge for the implications of the methods to develop this fungus as a microbial control agent. |
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