Occurrence of <Emphasis Type="Italic">Vibrio cholerae</Emphasis> non-O1 in three wastewater treatment plants in Agadir (Morocco) |
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Authors: | Rkia Eddabra Wardi Moussaoui Gilles Prévost François Delalande Alain Van Dorsselaer Olivier Meunier Jean-Michel Scheftel Rachida Mimouni |
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Institution: | 1.Laboratoire des Systèmes Aquatiques: Milieu Marin et Continental, Equipe: Ecotoxicologie et Microbiologie Appliquée, Faculté des Sciences,Université Ibn Zohr,Agadir,Morocco;2.UPRES EA-4438, Physiopathologie et Médecine Translationnelle, Laboratoire de Bactériologie de la Faculté de Médecine,Université Louis Pasteur,H?pitaux Universitaires de Strasbourg,France;3.INSERM U682, Développement et Physiopathologie de l’Intestin et du Pancréas,Université de Strasbourg,Strasbourg,France;4.Laboratoire d’Hygiène Hospitalière,H?pitaux Universitaires de Strasbourg,Strasbourg,France;5.Laboratoire de Spectrométrie de Masse BioOrganique, IPHC-DSA, ULP, CNRS,UMR7178,Strasbourg,France |
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Abstract: | A total of 21 isolates of Vibrio
cholerae non-O1 strains were isolated from three wastewater treatment plants in Agadir, Morocco. The isolates were analyzed by biochemical
analysis, antibiogram, pulsed-field gel electrophoresis and the MALDI-TOF patterns of their protein masses were compared.
Over 67% of isolates were susceptible to antimicrobial agents tested and 14% proved resistant to both trimethoprim-sulfamethoxazole
and nalidixic acid. Typing by pulsed-field gel electrophoresis with NotI digestion revealed that the V. cholerae non-O1 strains from Agadir (Morocco) have a lower level of genetic homogeneity, the restriction patterns of whole-chromosomal
DNA grouped the V. cholerae O1 and V. alginolyticus strains into a separate cluster from V. metschnikovii and V. cholerae non-O1 isolates. Furthermore, to gain additional analytical accuracy and reliability in the analysis we used dendrogram based
on MALDI-TOF spectral patterns generated by the BioTyper 1.1™ software. All m/z signatures of all strains tested indicate that the mass spectral data contained sufficient information to distinguish between
strains of V. cholerae. |
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